675 - Advanced or Special Topics in Cancer Research
Semester I, II; 1 to 3 credits. Various "minicourses" on a variety of topics have been offered under this title. Specific information is provided each semester.
The following courses are regular 675 offerings:
Contemporary Virology, Semester II; 3 credits. This course uses contemporary studies in Virology as a framework to help develop graduate student skills in data analysis and interpretation, proposal writing, and oral presentation. The first half of the course consists of a series of discussion sections in which review material and primary literature papers are discussed in depth. The primary papers have been selectively modified so that the students have to do much of the data interpretation for themselves with the help of a set of guide questions. Each section will end with the construction of a model that best explains the data. Also, future directions for the work will be proposed. The course also consists of a proposal workshop followed by a written proposal and an oral defense of that proposal. Prerequisites: Oncology 640; course is limited to first- and second-year graduate students. Instructor: Dan Loeb. Protein Purification, Semester I (odd-numbered years); 2 credits. It will be given
next in Fall 2007. It is primarily a lecture course consisting of 27 lectures,
2 half-semester exams, some take-home problems, and a paper on a topic relating
to the course. The students are asked to read at least twelve articles and
prepare an informative summary with references. Students are expected to read
"Protein Purification; Principles and Practice" Scopes, 3rd edition.
Goals of the course are: 1) to introduce the most important and
useful concepts of protein purification and handling, 2) to help students to
develop an intuition about how to work with proteins -- so that they can
"think like a protein", and 3) to guide students to ongoing sources of
information and resources.
Lecture topics include: Introduction-Protein purification overview;
Properties of proteins/types of separation methods; Assays -- following an
enzyme through a purification; Protein characterization; Protein inactivation
and stabilization/solution components; Purification strategy/starting
materials/preparing cell extracts; Precipitation methods; Computer simulation
of protein purification; Phase partitioning; Dialysis, desalting,
concentration, and ultrafiltration; Preparative electrophoresis,
chromatofocusing, isoelectric focusing, capillary electrophoresis;
Purification of membrane proteins/glycoproteins; Column chromatography --
theory and concepts; Sizing -- gel filtration chromatography; Ion exchange,
Affinity, Immunoaffinity, and DNA affinity chromatography; HPLC: Columns and
hardware, theory, methods development, applications; Micropurification by
eluting from SDS gels; Overproduction of cloned gene products; Purification
of insoluble overproduced proteins; Engineering proteins for ease of
purification and characterization; Recent advances in studying
protein-protein interactions; Immobilizing and using enzymes in bioreactors;
Scale-up considerations/related resources, courses, and facilities.
This course is usually taken for credit by about 25-30 graduate students, and
occasionally by senior undergraduates. Auditors or those who wish to sit in
on part or all of the lectures are welcome (usually as many as 20-30
specialists, graduate students, postdocs, and faculty sit in).
For more info contact: Dick Burgess - rburgess@wisc.edu
Statistical Problems in Genetics and Molecular Biology, Semester II (even-numbered years); 1 credit. Meets with Genetics 677 and Medical Genetics 677. This course is for scientists who generate data and want to be able to interpret their results. The emphasis is on the logic and probabilistic nature of statistics. Prerequisites: Consent of instructor. Instructors: Norman R. Drinkwater.
Appropriate Conduct and Effective Communication of Science, Semester II; 1 credit. A review and discussion of the fundamentals of good scientific communication and ethical issues in science. Instructors: Sally Leong and Michael Gould.
