William F. Dove, Ph.D.
George
Streisinger Professor Emeritus of Experimental Biology
Professor Emeritus of Oncology and Medical Genetics
A.B., 1958, Chemistry, Amherst College,
Massachusetts
Ph.D., 1962, Chemistry, California
Institute of Technology
Postdoctoral research: Cambridge
University; Stanford University
Office: 313A McArdle
Laboratory
Telephone: Office - (608) 262-4977; Lab -
(608) 262-0982
Email: dove@oncology.wisc.edu
Lab Home
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Research Interests: Colon cancer
Research Description: Our laboratory studies the genetic, cellular, and molecular interactions involved in cancer of the self-renewing mammalian intestinal epithelium. To pursue this goal, we have established two complementary animal models for familial colon cancer - the Min (Multiple intestinal neoplasia) mouse and Pirc (Polyposis in the rat colon) rat kindreds. By manipulating the genetic background of these kindreds, we can discover genes that quantitatively or qualitatively influence the colon cancer phenotype. The emergent power to analyze the genome, transcriptome, and proteome of the mouse, rat, and human connects these biological studies in experimental animals to the molecular players that control this disease in humans.
The power to phenotype the neoplastic process is greatly enhanced by achieving both spatial and temporal resolution. Spatial resolution by immunohistochemistry, in situ hybridization, or somatic lineage marking illuminates issues in cellular/genetic interactions involving the neoplastic lineage and the microenvironment. Imaging tumors over time in live animals by either optical endoscopy or virtual colonoscopy addresses issues in tumor progression and regression, spontaneous or induced by host factors, environmental agents, or drugs.
A doctoral or postdoctoral member of our laboratory would learn the biology of the laboratory mouse and rat, including the assay of molecular markers of neoplastic development by immunohistochemistry or in situ hybridization, objective assessment of neoplastic growth and regression, and investigation of the autonomy of gene action by tissue grafting, chimerism and mosaicism. Group members can broaden their research capacity through our ongoing research interactions with collaborators on campus. Working with biostatisticians, we become familiar with the genetics of quantitative modifiers of tumor susceptibility. Working with faculty in radiology and medical physics, we explore new imaging methods. With the Biotechnology Center, we engage the evolving power of mass spectrometry in the analysis of protein markers expressed in the plasma of tumor-bearing animals.
Altogether, uniquely complementing other investigations worldwide, our Wisconsin team hopes to achieve a deep understanding of the biology of colon cancer and thereby to impact its management in humans through diagnosis, prognosis, and early detection.
Selected recent publications
Washington, M. K., Powell, A. E., Sullivan, R., Sundberg, J. P., Wright, N., Coffey, R. J., and Dove, W. F. Pathology of Rodent Models of Intestinal Cancer: Progress Report and Recommendations. Gastroenterology, 144(4): 705-717, 2013.
Amos-Landgraf, J. M., Clipson, L., Newton, M. A., and Dove, W. F. The Many Ways to Open the Gate to Colon Cancer. Cell Cycle, 11: 1261-1262, 2012.
Amos-Landgraf, J. M., Irving, A. A., Hartman, C., Hunter, A., Laube, B., Chen, X., Clipson, L., Newton, M. A., and Dove, W. F. Monoallelic Silencing and Haploinsufficiency in Early Murine Intestinal Neoplasms. Proc. Natl. Acad. Sci. USA, 109: 2060-2065, 2012.
Dove, W., and Susman, M. Retrospective. James F. Crow (1916-2012). Science, 335: 812, 2012.
Thliveris, A. T., Clipson, L., Sommer, L. L., Schoenike, B. A., Hasenstein, J. R., Schlamp, C. L., Alexander, C. M., Newton, M. A., Dove, W. F., and Amos-Landgraf, J. M. Regulated Expression of Chromobox Homolog 5 Revealed in Tumors of ApcMin/+ ROSA11 Gene Trap Mice. G3 (Bethesda), 2: 569-578, 2012.
Boutwell, R. K., Burgess, R. R., Dove, W. F., and Pitot, H. C. On the Passing of Gerald C. Mueller, MD, PhD (1920-2010). Cancer Res., 71: 6909-6910, 2011.
Irving, A. A., Halberg, R. B., Albrecht, D. M., Plum, L. A., Krentz, K. J., Clipson, L., Drinkwater, N., Amos-Landgraf, J. M., Dove, W. F., and DeLuca, H. F. Supplementation by Vitamin D Compounds Does Not Affect Colonic Tumor Development in Vitamin D Sufficient Murine Models. Arch. Biochem. Biophys., 515: 64-71, 2011.
Thliveris, A. T., Clipson, L., White, A., Waggoner, J., Plesh, L., Skinner, B. L., Zahm, C. D., Sullivan, R., Dove, W. F., Newton, M. A., and Halberg, R. B. Clonal Structure of Carcinogen-Induced Intestinal Tumors in Mice. Cancer Prev. Res., 4: 916-923, 2011.