University of Wisconsin School of Medicine and Public Health McArdle Lab home

Henry C. Pitot, M.D., Ph.D.

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H. Pitot photo Professor Emeritus of Oncology and of Pathology and Laboratory Medicine

B.S., 1951, Chemistry, Virginia Military Institute
M.D., 1955, Medicine, Tulane University School of Medicine
Ph.D., 1959, Biochemistry, Tulane University School of Medicine
Postdoctoral research: McArdle Laboratory, University of Wisconsin-Madison

Office: 522 McArdle Laboratory
Telephone: Office - (608) 262-3247
Email: pitot@oncology.wisc.edu


Selected recent publications

Cleveland, A. G., Oikarinen, S. I., Bynoté, K. K., Marttinen, M., Rafter, J. J., Gustafsson, J.-Å., Roy, S. K., Pitot, H. C., Korach, K. S., Lubahn, D. B., Mutanen, M., and Gould, K. A.  Disruption of Estrogen Receptor Signaling Enhances Intestinal Neoplasia in ApcMin/+ Mice.  Carcinogenesis, 30:  1581-1590, 2009.

Halberg, R. B., Waggoner, J., Rasmussen, K., White, A., Clipson, L., Prunuske, A. J., Bacher, J. W., Sullivan, R., Washington, M. K., Pitot, H. C.  Petrini, J. H. J., Albertson, D. G., and Dove, W. F.  Long-lived Min Mice Develop Advanced Intestinal Cancers through a Genetically Conservative Pathway.  Cancer Res., 69:  5768-5775, 2009.

Peychal, S. E.-M., Bilger, A., Pitot, H. C., and Drinkwater, N. R.  Predominant Modifier of Extreme Liver Cancer Susceptibility in C57BR/cdJ Female Mice Localized to 6 Mb on Chromosome 17.  Carcinogenesis, 30: 879-885, 2009.

Decker, N. K., Abdelmoneim, S. S., Yaqoob, U., Hendrickson, H., Hormes, J., Bentley, M., Pitot, H., Urrutia, R., Gores, G. J., and Shah, V. H.  Nitric Oxide Regulates Tumor Cell Cross-Talk with Stromal Cells in the Tumor Microenvironment of the Liver.  Am. J. Pathol., 173:  1002-1012, 2008.

Halberg, R. B., Chen, X., Amos-Landgraf, J. M., White, A., Rasmussen, K., Clipson, L., Pasch, C., Sullivan, R., Pitot, H. C., and Dove, W. F.  The Pleiotropic Phenotype of Apc Mutations in the Mouse:  Allele Specificity and Effects of the Genetic Background.  Genetics, 180:  601-609, 2008.

Shai, A., Pitot, H. C., and Lambert, P. F.  p53 Loss Synergizes with Estrogen and Papillomaviral Oncogenes to Induce Cervical and Breast Cancers.  Cancer Res., 68:  2622-2631, 2008.

Czarny, M. J., Babcock, K., Baus, R. M., Manoharan, H., and Pitot, H. C.  Hepatocellular Carcinomas of the Albumin SV40 T-Antigen Transgenic Rat Display Fetal-Like Re-Expression of Igf2 and Deregulation of H19.  Mol. Carcinog., 46:  747-757, 2007.

Maufort, J. P., Genther Williams, S. M., Pitot, H. C., and Lambert, P. F.  Human Papillomavirus 16 E5 Oncogene Contributes to Two Stages of Skin Carcinogenesis.  Cancer Res., 67:  6106-6112, 2007.

Pitot, H. C. Adventures in Hepatocarcinogenesis. Annu. Rev. Pathol. Mech. Dis., 2: 1-29, 2007.

Pitot, H. C.  On Being a Pathologist.  Hum. Pathol., 38: 824-829, 2007.

Xu, Y.-H., Manoharan, H. T., and Pitot, H. C.  CpG PatternFinder:  a Windows®-based Utility Program for Easy and Rapid Identification of the CpG Methylation Status of DNA.  BioTechniques, 43:  334-342, 2007.

Holsapple, M. P., Pitot, H. C., Cohen, S. H., Boobis, A. R., Klaunig, J. E., Pastoor, T., Dellarco, V. L., and Dragan, Y. P. Mode of Action in Relevance of Rodent Liver Tumors to Human Cancer Risk. Toxicol. Sci., 89: 51-56, 2006.

Ogawa, H., Gomi, T., Nishizawa, M., Hayakawa, Y., Endo, S., Hayashi, K., Ochiai, H., Takusagawa, F., Pitot, H. C., Mori, H., Sakurai, H., Koizumi, K., Saiki, I., Oda, H., Fujishita, T., Miwa, T., Maruyama, M., and Kobayashi, M.  Enzymatic and Biochemical Properties of a Novel Human Serine Dehydratase Isoform.  Biochim. Biophys. Acta, 1764:  961-971, 2006.

Strati, K., Pitot, H. C., and Lambert, P. F.  Identification of Biomarkers That Distinguish Human Papillomavirus (HPV)-positive Versus HPV-negative Head and Neck Cancers in a Mouse Model.  Proc. Natl. Acad. Sci. USA, 103:  14152-14157, 2006.

Xu, Y., and Pitot, H. C. A Software Package to Improve Image Quality and Isolation of Objects of Interest for Quantitative Stereology Studies of Rat Hepatocarcinogenesis. Comput. Methods Programs Biomed., 81: 236-245, 2006.

Fang, H., Tong, W., Perkins, R., Shi, L., Hong, H., Cao, X., Xie, Q., Yim, S. H., Ward, J. M., Pitot, H. C., and Dragan, Y. P. Bioinformatics Approaches for Cross-species Liver Cancer Analysis Based on Microarray Gene Expression Profiling. BMC Bioinformatics, 6(Suppl 2):S6, 2005.

Kashii, T., Gomi, T., Oya, T., Ishii, Y., Oda, H., Maruyama, M., Kobayashi, M., Masuda, T., Yamazaki, M., Nagata, T., Tsukada, K., Nakajima, A., Tatsu, K., Mori, H., Takusagawa, F., Ogawa, H., and Pitot, H. C. Some Biochemical and Histochemical Properties of Human Liver Serine Dehydratase. Int. J. Biochem. Cell Biol., 37: 574-589, 2005.

Lahvis, G. P., Pyzalski, R. W., Glover, E., Pitot, H. C., McElwee, M. K., and Bradfield, C. A. The Aryl Hydrocarbon Receptor Is Required for Developmental Closure of the Ductus Venosus in the Neonatal Mouse. Mol. Pharmacol., 67: 714-720, 2005.

Xu, Y.-H., Manoharan, H. T., and Pitot, H. C. CpG Analyzer, a Windows-based Utility Program for Investigation of DNA Methylation. Biotechniques, 39: 656-662, 2005.

Bilger, A., Bennett, L. M., Carabeo, R. A., Chiaverotti, T. A., Dvorak, C., Liss, K. M., Schadewald, S. A., Pitot, H. C., and Drinkwater, N. R. A Potent Modifier of Liver Cancer Risk on Distal Mouse Chromosome 1: Linkage Analysis and Characterization of Congenic Lines. Genetics, 167: 859-866, 2004.

Dragan, Y. P., Sargent, L. M., Babcock, K., Kinunen, N., and Pitot, H. C. Alterations in Specific Gene Expression and Focal Neoplastic Growth during Spontaneous Hepatocarcinogenesis in Albumin-SV40 T Antigen Transgenic Rats. Mol. Carcinog., 40: 150-159, 2004.

Manoharan, H., Babcock, K., and Pitot, H. C. Changes in the DNA Methylation Profile of the Rat H19 Gene Upstream Region During Development and Transgenic Hepatocarcinogenesis and Its Role in the Imprinted Transcriptional Regulation of the H19 Gene. Mol. Carcinog., 41: 1-16, 2004.

Tessier, C. R., Doyle, G. A., Clark, B. A., Pitot, H. C., and Ross, J. Mammary Tumor Induction in Transgenic Mice Expressing an RNA-Binding Protein. Cancer Res., 64: 209-214, 2004.

Xu, Y.-H., Lahvis, G., Edwards, H., and Pitot, H. C. Three-dimensional Reconstruction from Serial Sections in PC-Windows Platform by Using 3D_Viewer. Comput. Methods Programs Biomed., 76: 143-154, 2004.

Boivin, G. P., Washington, K., Yang, K., Ward, J. M., Pretlow, T. P., Russell, R., Besselsen, D. G., Godfrey, V. L., Doetschman, T., Dove, W. F., Pitot, H. C., Halberg, R. B., Itzkowitz, S. H., Groden, J., and Coffey, R. J. Pathology of Mouse Models of Intestinal Cancer: Consensus Report and Recommendations. Gastroenterology, 124: 762-777, 2003.

Manoharan, H., Babcock, K., Willi, J., and Pitot, H. C. Biallelic Expression of the H19 Gene During Spontaneous Hepatocarcinogenesis in the Albumin SV40 T Antigen Transgenic Rat. Mol. Carcinog., 38: 40-47, 2003.

Masuda, T., Ogawa, H., Matsushima, T., Kawamata, S., Sasahara, M., Kuroda, K., Suzuki, Y., Takata, Y., Yamazaki, M., Takusagawa, F., and Pitot, H. C. Localization and Hormonal Control of Serine Dehydratase during Metabolic Acidosis Differ Markedly From Those of Phosphoenolpyruvate Carboxykinase in Rat Kidney. Int. J. Biochem. Cell Biol., 35: 1234-1247, 2003.

Ou, Y. C., Conolly, R. B., Thomas, R. S., Gustafson, D. L., Long, M. E., Dobrev, I. D., Chubb, L. S., Xu, Y., Lapidot, S. A., Andersen, M. E., and Yang, R. S. H. Stochastic Simulation of Hepatic Preneoplastic Foci Development for Four Chlorobenzene Congeners in a Medium-Term Bioassay. Toxicol. Sci., 73: 301-314, 2003.

Xu, Y.-H., and Pitot, H. C. An Improved Stereologic Method for Three-Dimensional Estimation of Particle Size Distribution from Observations in Two Dimensions and Its Application. Computer Methods Programs Biomed., 72: 1-20, 2003.

Yamada, T., Komoto, J., Takata, Y., Ogawa, H., Pitot, H. C., and Takusagawa, F. Crystal Structure of Serine Dehydratase from Rat Liver. Biochemistry, 42: 12854-12865, 2003.

Pitot, H. C. Fundamentals of Oncology, 4th edition, 998 pp. New York: Marcel Dekker, Inc., 2002.

Divi, R. L., Dragan, Y. P., Pitot, H. C., and Poirier, M. C. Immunohistochemical Localization and Semi-quantitation of Hepatic Tamoxifen-DNA Adducts in Rats Exposed Orally to Tamoxifen. Carcinogenesis, 22: 1693-1699, 2001.

Dragan, Y. P., Nuwaysir, E., Sargent, L., Li, D.-H., Jordan, V. C., and Pitot, H. C. The Effects of Triphenylethylene Antiestrogens on Parameters of Multistage Hepatocarcinogenesis in the Rat. In: J. J. Li, S. A. Li, and J. R. Daling (Eds.), Hormonal Carcinogenesis III. Proceedings of the Third International Symposium, Chapter 9, pp. 124-135. New York: Springer-Verlag, 2001.

Hall, D. M., Sattler, G. L., Sattler, C. A., Zhang, H. J., Oberley, L. W., Pitot, H. C., and Kregel, K. C. Aging Lowers Steady-State Antioxidant Enzyme and Stress Protein Expression in Primary Hepatocytes. J. Gerontol.: Biol. Sci., 56A: B259-B267, 2001.

Ou, Y. C., Conolly, R. B., Thomas, R. S., Xu, Y., Andersen, M. E., Chubb, L. S., Pitot, H. C., and Yang, R. S. H. A Clonal Growth Model: Time-course Simulations of Liver Foci Growth following Penta- or Hexachlorobenzene Treatment in a Medium-term Bioassay. Cancer Res., 61: 1879-1889, 2001.

Pitot, H. C. Animal Models of Neoplastic Development. Dev. Biol., 106: 53-59, 2001.

Pitot, H. C. Pathways of Progression in Hepatocarcinogenesis. Lancet, 358: 859-860, 2001.

Pitot III, H. C., and Dragan, Y. P. Chemical Carcinogenesis. In: C. D. Klaassen (Ed.), Casarett and Doull's Toxicology. The Basic Science of Poisons, Sixth Ed., Chapter 8, pp. 241-319. New York, NY: McGraw-Hill Medical Publishing Division, 2001.

Dragan, Y., Valdés, R., Gomez-Angelats, M., Felipe, A., Casado, F. J., Pitot, H., and Pastor-Anglada, M. Selective Loss of Nucleoside Carrier Expression in Rat Hepatocarcinomas. Hepatology, 32: 239-246, 2000.

Firozi, P. F., Vulimiri, S. V., Rajaniemi, H., Hemminki, K., Dragan, Y., Pitot, H. C., DiGiovanni, J., Zhu, Y.-H., and Li, D. Characterization of the Major DNA Adducts in the Liver of Rats Chronically Exposed to Tamoxifen for 18 Months. Chem.-Biol. Interact., 126: 33-43, 2000.

Haas, M. J., Sattler, C. A., Dragan, Y. P., Gast, W. L., and Pitot, H. C. Multiple Polypeptide Hormone Expression in Pancreatic Islet Cell Carcinomas Derived from Phosphoenolpyruvatecarboxykinase-SV40 T Antigen Transgenic Rats. Pancreas, 20: 206-214, 2000.

Pitot, H. C., Hikita, H., Dragan, Y., Sargent, L., and Haas, M. Review Article: The Stages of Gastrointestinal Carcinogenesis - Application of Rodent Models to Human Disease. Aliment. Pharmacol. Ther., 14(Suppl. 1): 153-160, 2000.

Teeguarden, J. G., Dragan, Y., and Pitot, H. C. Hazard Assessment of Chemical Carcinogens: the Impact of Hormesis. J. Appl. Toxicol., 20: 113-120, 2000.

Teeguarden, J. G., Newton, M. A., Dragan, Y. P., and Pitot, H. C. Genome-Wide Loss of Heterozygosity Analysis of Chemically Induced Rat Hepatocellular Carcinomas Reveals Elevated Frequency of Allelic Imbalances on Chromosomes 1, 6, 8, 11, 15, 17, and 20. Mol. Carcinog., 28: 51-61, 2000.

Xu, Y.-H., Sattler, G. L., Edwards, H., and Pitot, H. C. Nuclear-Labeling Index Analysis (NLIA), a Software Package Used to Perform Accurate Automation of Cell Nuclear-Labeling Index Analysis on Immunohistochemically Stained Rat Liver Samples. Comput. Methods Programs Biomed., 63: 55-70, 2000.

Gómez-Angelats, M., Teeguarden, J. G., Dragan, Y. P., and Pitot, H. C. Mutational Analysis of Three Tumor Suppressor Genes in Two Models of Rat Hepatocarcinogenesis. Mol. Carcinog., 25: 157-163, 1999.

Haas, M. J., Dragan, Y. P., Hikita, H., Shimel, R., Takimoto, K., Heath, S., Vaughan, J., and Pitot, H. C. Transgene Expression and Repression in Transgenic Rats Bearing the Phosphoenolpyruvate Carboxykinase-Simian Virus 40 T Antigen or the Phosphoenolpyruvate Carboxykinase-Transforming Growth Factor-a Constructs. Am. J. Pathol., 155: 183-192, 1999.

Haas, M. J., and Pitot, H. C. Glucocorticoids Stimulate CREB Binding to a Cyclic-AMP Response Element in the Rat Serine Dehydratase Gene. Arch. Biochem. Biophys., 362: 317-324, 1999.

Hikata, H., Vaughan, J., Babcock, K., and Pitot, H. C. Short-Term Fasting and the Reversal of the Stage of Promotion in Rat Hepatocarcinogenesis: Role of Cell Replication, Apoptosis, and Gene Expression. Toxicol. Sci., 52(Suppl.): 17-23, 1999.

Li, D., Wang, M., Paul, G., Pitot, H. C., and Dragan, Y. P. Dietary Oat Lipids-Induced Novel DNA Modifications and Suppression of Altered Hepatic Foci Formation. Nutr. Cancer, 33: 40-45, 1999.

Ogawa, H., Takusagawa, F., Wakaki, K., Kishi, H., Eskandarian, M. R., Kobayashi, M., Date, T., Huh, N.-H., and Pitot, H. C. Rat Liver Serine Dehydratase. Bacterial Expression and Two Folding Domains as Revealed by Limited Proteolysis. J. Biol. Chem., 274: 12855-12860, 1999.

Song, S., Pitot, H. C., and Lambert, P. F. The Human Papillomavirus Type 16 E6 Gene Alone Is Sufficient To Induce Carcinomas in Transgenic Animals. J. Virol., 73: 5887-5893, 1999.

Teeguarden, J. G., Dragan, Y. P., Singh, J., Vaughan, J., Xu, Y.-H., Goldsworthy, T., and Pitot, H. C. Quantitative Analysis of Dose- and Time-Dependent Promotion of Four Phenotypes of Altered Hepatic Foci by 2,3,7,8-Tetrachlorodibenzo-p-dioxin in Female Sprague-Dawley Rats. Toxicol. Sci., 51: 211-223, 1999.

Xu, Y.-H., and Pitot, H. C. Building Quantitative Stereology Data Files with Scion Image, a Public Domain Image Processing and Analysis Software. Comput. Methods Programs Biomed., 59: 131-142, 1999.

Bulera, S. J., Sattler, C. A., Gast, W. L., Heath, S., Festerling, T. A., and Pitot, H. C. The Mechanism of Thioacetamide-induced Apoptosis in the L37 Albumin-SV40 T-Antigen Transgenic Rat Hepatocyte-derived Cell Line Occurs Without DNA Fragmentation. In Vitro Cell. Dev. Biol.-Anim., 34: 685-693, 1998.

Dragan, Y., Klaunig, J., Maronpot, R., and Goldsworthy, T. Mechanisms of Susceptibility to Mouse Liver Carcinogenesis. Toxicol. Sci., 41: 3-7, 1998.

Dragan, Y. P., Shimel, R. J., Bahnub, N., Sattler, G., Vaughan, J. R., Jordan, V. C., and Pitot, H. C. Effect of Chronic Administration of Mestranol, Tamoxifen, and Toremifene on Hepatic Ploidy in Rats. Toxicol. Sci., 43: 129-138, 1998.

Gohda, E., and Pitot, H. C. Cathepsin T. In: A. J. Barrett, N. D. Rawlings, J. F. Woessner (Eds.), Handbook of Proteolytic Enzymes, pp. 774-775. San Diego: Academic Press, 1998.

Haas, M. J., and Pitot, H. C. Characterization of Rare p53 Mutants from Carcinogen-treated Albumin-Simian Virus 40 T-Antigen Transgenic Rats. Mol. Carcinog., 21: 128-134, 1998.

Hikita, H., Nuwaysir, E. F., Vaughan, J., Babcock, K., Haas, M. J., Dragan, Y. P., and Pitot, H. C. The Effect of Short-term Fasting, Phenobarbital and Refeeding on Apoptotic Loss, Cell Replication and Gene Expression in Rat Liver during the Promotion Stage. Carcinogenesis, 19: 1417-1425, 1998.

Nuwaysir, E. F., Dragan, Y. P., McCague, R., Martin, P., Mann, J., Jordan, V. C., and Pitot, H. C. Structure-Activity Relationships for Triphenylethylene Antiestrogens on Hepatic Phase-I and Phase-II Enzyme Expression. Biochem. Pharmacol., 56: 321-327, 1998.

O'Regan, R. M., Cisneros, A., England, G. M., MacGregor, J. I., Muenzner, H. D., Assikis, V. J., Bilimoria, M. M., Piette, M., Dragan, Y. P., Pitot, H. C., Chatterton, R., and Jordan, V. C. Effects of the Antiestrogens Tamoxifen, Toremifene, and ICI 182,780 on Endometrial Cancer Growth. J. Natl. Cancer Inst., 90: 1552-1558, 1998.

Pitot, H. C. Hepatocyte Death in Hepatocarcinogenesis. Hepatology, 28: 1-5, 1998.

Pitot, H. C. The Progression of Neoplasia, Cell Replication, and Electromagnetic Fields. Int. J. Toxicol., 17(Suppl 3): 59-108, 1998.

Steinmetz, K. L, Pogribny, I. P., James, S. J., and Pitot, H. C. Hypomethylation of the Rat Glutathione S-Transferase p (GSTP) Promoter Region Isolated from Methyl-deficient Livers and GSTP-positive Liver Neoplasms. Carcinogenesis, 19: 1487-1494, 1998.

Teeguarden, J. G., Dragan, Y. P., and Pitot, H. C. Implications of Hormesis on the Bioassay and Hazard Assessment of Chemical Carcinogens. Hum. Exp. Toxicol., 17: 254-258, 1998.

Teeguarden, J. G., Dragan, Y., and Pitot, H. C. Implications of Hormesis on the Bioassay and Hazard Assessment of Chemical Carcinogens. BELLE Newsletter, 6: 8-13, 1998.

Xu, Y.-H., Dragan, Y. P., Campbell, H. A., and Pitot, H. C. STEREO: A Program on a PC-Windows 95 Platform for Recording and Evaluating Quantitative Stereologic Investigations of Multistage Hepatocarcinogenesis in Rodents. Comput. Methods Programs Biomed., 56: 49-63, 1998.