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Research Interests: Novel methods for genome mapping and sequencing

Research Description: The objectives of our research on various aspects of genomics, including gene cloning, architecture and expression have been:
 (1) developing new methods for (i) ordering and in situ amplification of 30- to 100-kb fragments of large eukaryotic genomes, without conventional cloning, and (ii) ordered and automated sequencing of such large fragments by employing our SPEL-6 technique, based on primer assembly by hexamer ligation and primer walking;
(2) developing improved derivatives of pBAC vectors allowing conditional in vivo excision and amplification of 3- to 10-kb (cDNA) or 30- to 200 kb (genomic DNA) inserts in E. coli hosts [for more details, please see this abstract];
(3) the design and construction of novel regulatory circuits;
(4) the cloning of viral genes with dominant-lethal mutations as the basis for developing a new kind of immunity control against viral infection of plants and other organisms; and
(5) modifying and increasing specificity of restriction enzymes.

Selected recent publications

Wild, J., and Szybalski, W. Copy-Control pBAC/oriV Vectors for Genomic Cloning. In: P. Balbas and A. Lorence (Eds.), Methods in Molecular Biology, Recombinant Gene Expression. Reviews and Protocols, Vol. 267, Chap. 10, pp. 145-154. Humana Press, Inc., 2004.

Wild, J., and Szybalski, W. Copy-Control Tightly Regulated Expression Vectors Based on pBAC/oriV. In: P. Balbas and A. Lorence (Eds.), Methods in Molecular Biology, Recombinant Gene Expression. Reviews and Protocols, Vol. 267, Chap. 11, pp. 155-167. Humana Press, Inc., 2004.

Roberts, R. J., Belfort, M., Bestor, T., Bhagwat, A. S., Bickle, T. A., Bitinaite, J., Blumenthal, R. M., Degtyarev, S. Kh., Dryden, D. T. F., Dybvig, K., Firman, K., Gromova, E. S., Gumport, R. I., Halford, S. E., Hattman, S., Heitman, J., Hornby, D. P., Janulaitis, A., Jeltsch, A., Josephsen, J., Kiss, A., Klaenhammer, T. R., Kobayashi, I., Kong, H., Krüger, D. H., Lacks, S., Marinus, M. G., Miyahara, M., Morgan, R. D., Murray, N. E., Nagaraja, V., Piekarowicz, A., Pingoud, A., Raleigh, E., Rao, D. N., Reich, N., Repin, V. E., Selker, E. U., Shaw, P.-C., Stein, D. C., Stoddard, B. L., Szybalski, W., Trautner, T. A., Van Etten, J. L., Vitor, J. M. B., Wilson, G. G., and Xu, S.-y. A Nomenclature for Restriction Enzymes, DNA Methyltransferases, Homing Endonucleases and Their Genes. Nucleic Acids Res., 31: 1805-1812, 2003.

Szybalski, W. Recollections of 1939-1949: From Politechnika Lwowska to Politechnika Gdańska. Acta Biochim. Pol., 50: XVII-XXI, 2003.

Wild, J., Hradecna, Z., and Szybalski, W. Conditionally Amplifiable BACs: Switching From Single-Copy to High-Copy Vectors and Genomic Clones. Genome Res., 12: 1434-1444, 2002.

Sektas, M., Hasan, N., and Szybalski, W. Expression Plasmid with a Very Tight Two-Step Control: Int/att-mediated Gene Inversion with Respect to the Stationary Promoter. Gene, 267: 213-220, 2001.

Szybalski, W. Perspectives: My Road to Øjvind Winge, the Father of Yeast Genetics. Genetics, 158: 1-6, 2001.

Wild, J., Hradecna, Z., and Szybalski, W. Single-Copy/High-Copy (SC/HC) pBAC/oriV Novel Vectors for Genomics and Gene Expression. Plasmid, 45: 142-149, 2001.

Palmeros, B., Wild, J., Szybalski, W., LeBorgne, S., Hernández-Chávez, G., Gosset, G., Valle, F., and Bolivar, F. A Family of Removable Cassettes Designed to Obtain Antibiotic-Resistance-free Genomic Modifications of Escherichia coli and Other Bacteria. Gene, 247: 255-264, 2000.

Szybalski, W. In Memoriam: Alfred D. Hershey (1908-1997). In: F. W. Stahl (Ed.), We Can Sleep Later. Alfred D. Hershey and the Origins of Molecular Biology, pp. 19-22. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press, 2000.

Kaczorowski, T., and Szybalski, W. Genomic DNA Sequencing by SPEL-6 Primer Walking using Hexamer Ligation. Gene, 223: 83-91, 1998.

Szybalski, W. Karol Taylor (1928-1997) - In Memoriam. Gene, 223: 393-409, 1998.

Wild, J., Sektas, M., Hradecna, Z., and Szybalski, W.  Targeting and Retrofitting Pre-Existing Libraries of Transposon Insertions with FRT and oriV Elements for in Vivo Generation of Large Quantities of Any Genomic Fragment.  Gene, 223: 55-66, 1998.

Yoon, Y. G., Pósfai, G., Szybalski, W., and Kim, S. C.  Cre/loxP-mediated in Vivo Excision of Large Segments From Yeast Genome and Their Amplification Based on the 2 mm Plasmid-derived System.  Gene, 223: 67-76, 1998.

Szybalski, W.  RecA-mediated Achilles' Heel Cleavage.  Curr. Opin. Biotechnol., 8: 75-81, 1997.

A complete list of Publications is available.