Waclaw Szybalski, D.Sc.

Professor Emeritus of Oncology

Ch.Eng., 1944, Chemistry, Institute of Technology, Lwów, Poland
M.Ch.Eng., 1945, Chemical Engineering, Institute of Technology, Gliwice, Poland
D.Sc., 1949, Microbial Biochemistry, Institute of Technology, Gdansk, Poland

Office: 618A McArdle Laboratory
Telephone: Office - (608) 262-1259; Lab - (608) 262-2047
Email: szybalski@oncology.wisc.edu

Research Interests: Novel methods for genome mapping and sequencing

Research Description: The objectives of our research on various aspects of genomics, including gene cloning, architecture and expression have been:
 (1) developing new methods for (i) ordering and in situ amplification of 30- to 100-kb fragments of large eukaryotic genomes, without conventional cloning, and (ii) ordered and automated sequencing of such large fragments by employing our SPEL-6 technique, based on primer assembly by hexamer ligation and primer walking;
(2) developing improved derivatives of pBAC vectors allowing conditional in vivo excision and amplification of 3- to 10-kb (cDNA) or 30- to 200 kb (genomic DNA) inserts in E. coli hosts [for more details, please see this abstract];
(3) the design and construction of novel regulatory circuits;
(4) the cloning of viral genes with dominant-lethal mutations as the basis for developing a new kind of immunity control against viral infection of plants and other organisms; and
(5) modifying and increasing specificity of restriction enzymes.

Selected recent publications

Lobocka, M., and Szybalski, W. T.  Preface.  Adv. Virus Res., 82:  xiii-xv, 2012.

Malecki, M., and Szybalski, W.  Isolation of Single, Intact Chromosomes from Single, Selected Ovarian Cancer Cells for in Situ Hybridization and Sequencing.  Gene, 493: 132-139, 2012.

Kakirde, K. S., Wild, J., Godiska, R., Mead, D. A., Wiggins, A. G., Goodman, R. M., Szybalski, W., and Liles, M. R.  Gram Negative Shuttle BAC Vector for Heterologous Expression of Metagenomic Libraries.  Gene, 475: 57-62, 2011.

Méndez-Lago, M., Wild, J., Whitehead, S. L., Tracey, A., de Pablos, B., Rogers, J., Szybalski, W., and Villasante, A.  Novel Sequencing Strategy for Repetitive DNA in a Drosophila BAC Clone Reveals That the Centromeric Region of the Y Chromosome Evolved from a Telomere.  Nucleic Acids Res., 37: 2264-2273, 2009.

Wild, J., and Szybalski, W.  Copy-Control pBAC/oriV Vectors for Genomic Cloning.  In:  P. Balbas and A. Lorence (Eds.), Methods in Molecular Biology, Recombinant Gene Expression.  Reviews and Protocols, Vol. 267, Chap. 10, pp. 145-154.  Humana Press, Inc., 2004.

Wild, J., and Szybalski, W.  Copy-Control Tightly Regulated Expression Vectors Based on pBAC/oriV.  In:  P. Balbas and A. Lorence (Eds.), Methods in Molecular Biology, Recombinant Gene Expression.  Reviews and Protocols, Vol. 267, Chap. 11, pp. 155-167.  Humana Press, Inc., 2004.

Roberts, R. J., Belfort, M., Bestor, T., Bhagwat, A. S., Bickle, T. A., Bitinaite, J., Blumenthal, R. M., Degtyarev, S. Kh., Dryden, D. T. F., Dybvig, K., Firman, K., Gromova, E. S., Gumport, R. I., Halford, S. E., Hattman, S., Heitman, J., Hornby, D. P., Janulaitis, A., Jeltsch, A., Josephsen, J., Kiss, A., Klaenhammer, T. R., Kobayashi, I., Kong, H., Krüger, D. H., Lacks, S., Marinus, M. G., Miyahara, M., Morgan, R. D., Murray, N. E., Nagaraja, V., Piekarowicz, A., Pingoud, A., Raleigh, E., Rao, D. N., Reich, N., Repin, V. E., Selker, E. U., Shaw, P.-C., Stein, D. C., Stoddard, B. L., Szybalski, W., Trautner, T. A., Van Etten, J. L., Vitor, J. M. B., Wilson, G. G., and Xu, S.-y.  A Nomenclature for Restriction Enzymes, DNA Methyltransferases, Homing Endonucleases and Their Genes.  Nucleic Acids Res., 31:  1805-1812, 2003.

Szybalski, W.  Recollections of 1939-1949:  From Politechnika Lwowska to Politechnika Gdanska.  Acta Biochim. Pol., 50:  XVII-XXI, 2003.

Wild, J., Hradecna, Z., and Szybalski, W.  Conditionally Amplifiable BACs:  Switching from Single-Copy to High-Copy Vectors and Genomic Clones.  Genome Res., 12:  1434-1444, 2002.

Szybalski, W.  Perspectives:  My Road to Øjvind Winge, the Father of Yeast Genetics.  Genetics, 158:  1-6, 2001.

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A complete list of Publications is available.